Structure of E. coli 16S RNA elucidated by psoralen crosslinking.

Structure-function relations in E. coli 16S RNA.

As a more thorough understanding of RNA secondary and tertiary structure has developed over the last several years, so has an appreciation of its importance in the function of the various RNP particles of which it is a part. For example, Ul RNA has been linked to mRNA splicing (Lerner et al., 1980; Rogers and Wall, 1980) while the RNA moiety of RNAase P has been shown to be absolutely required ...

Mapping the location of psoralen crosslinks on RNA by mung bean nuclease sensitivity of RNADNA hybrids ( ribosomal RNA / Shine - Dalgarno sequence / RNA secondary structure / nucleotide sequence )

An indirect high resolution method has been developed for finding the location of intrastrand crosslinks in RNA. An end-labeled DNA strand that overlaps the approximate crosslink position is hybridized to the RNA and then treated with mung bean nuclease. The resulting digest is analyzed on a sequencing-type gel. The method was tested with the major psoralen crosslink seen in the 16S rRNA of ina...

Photochemical crosslinking of transcription complexes with psoralen. I. Covalent attachment of in vitro SV40 nascent RNA to its double-stranded DNA template.

14C-labeled SV40 DNA has been transcribed with E. coli RNA polymerase using 3H-labeled ribonucleotide triphosphates as precursors. The resulting transcription complexes were then photochemically crosslinked with the psoralen derivative, 4'-aminomethyl-4,5', 8-trimethylpsoralen (AMT), at 37 degrees C and analyzed in SDS-sucrose gradients. It was found that the photochemical crosslinking procedur...

Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh.

Radioactively labeled 4.5S RNA containing statistically distributed 4-thiouridine residues in place of normal uridine was prepared by T7 transcription. The ability of this modified 4.5S RNA to form a complex with the protein Ffh was demonstrated by a gel shift assay. The modified 4.5S RNA, with or without Ffh, was added to Escherichia coli ribosomes under various conditions, and crosslinking fr...

Genes Affecting the Repair and Survival of Escherichia coli Following Psoralen-Induced Damage: a DNA Interstrand Crosslinking Agent by Anthonige